COURSE DETAILS


Short Course for Biological Scanning Electron Microscopy


The course is designed for the beginner who has no prior experience in the field, and is interested in utilizing the SEM for their research project. On completion, participants should be able to prepare and examine samples by themselves.


Total class hours : 2.5 hr. lecture, 6 hr. demonstration and group practice
Fee : $125.00 (materials & lab manual included)
Time : 4 consecutive afternoon sessions or one session per week for 4 weeks

Content Covered:
* Basic theory of SEM and applications
*Introduction to the use of SEM (Hitachi S-2500)
*Specimen preparation
*3-D image & low voltage image
*Hands on practice:
astigmatism correction, change Kv and working distance, photography, etc.
*2 hours SEM operation for individual practice after course (by appointment)

For registration, please call Dr. Chen at (403) 492-3621 or e-mail at mingchen@gpu.srv.ualberta.ca

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Some of Dr. Chen's Students that have taken the SEM course.


Basic Biological Transmission Electron Microscopy


The course aims to familiarize participants with the capabilities of biological transmission electron microscopy. On completion, participants should be able to prepare and examine tissue following simple conventional protocols.


Total class hours : 4 hr. lectures, 6 hr. demonstrations and hands on practice
Fee: $150:00 (material and lab manual included)
Time : By arrangement, either one session per week or two sessions per week


Content covered:
*Lectures will cover basic electron optics, fixation, embedding, ultrathin sectioning and staining, immuno-cytochemistry EM and darkroom techniques
*TEM observation including filament saturation, objective aperture selection, astigmatism correction, specimen exchange and photography etc..
*Individual hands-on practice in your own suitable time for glassknife making , microtom techniques and EM observation (by appointment)
*Certificate will be issued after the completion of assignments.

For registration, please call Dr. Chen at (403) 492-3621 or e-mail at mingchen@gpu.srv.ualberta.ca


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