Equipment List
MICROSCOPES AND IMAGE ANALYSIS:
Olympus Microscope System (Model BX40) with Reflected Light Fluorescence Attachment (Model BX-FLA)
Olympus BX40 Microscope attached with BX-FLA reflected light fluorescence vertical illuminator has 4X, 10X, 20X, 40X and 100X Plan Fluorite “universal” objectives and three modular filter cubes for
a) blue image, UV exciter filter BP 360-370 /bandpass barrier filter BA 420 - 460;
b) green image, blue exciter filter BP 460 - 490 /bandpass barrier filter BA 515 -550; and
c) red image, green exciter filter BP 530 -550 /bandpass barrier filter BA 570 -590.
Leica Inverted Microscope System (Model DM IRB) with Reflected Light Fluorescence Attachment (Model RL)
This DM IRB inverted microscope has objective magnifications from 5x, 10x, 20x and 40x (new). Three fluorescence filter systems for blue, green and red images are accommodated on this microscope (please read BX40 microscope for detail of excitation filters and barrier filters).
Image Analysis System
Image analysis system includes a SPOT2 color digital camera and image analysis software (Image-Pro Plus v4.5). The SPOT CCD chip has a resolution of 1315 x 1033 pixels (total 1,358,395 pixels). This is more than 4 times the resolution of a 3CCD camera with a 640 x 480 frame grabber (307,200 pixels). By exposing the CCD three times (once each for red, green and blue), this camera is able to get true and accurate values for each color on every CCD pixel. Image-Pro Plus allows you to enhance your image quality and to analyze your image in several ways such as intensity analysis, measuring and counting and data exchange.
Related Protocols:
Immunohistochemistry (IHC):
IHC- Paraffin Embedded Tissues
Immunofluorescence (IF)
IF-Cells
IF-Frozen Tissues
IF-Paraffin Embedded Tissues
IF-Autofluoresence Control
Fluoresence-labeled In situ Hybridization (FISH):
FISH-Cells
Count Staining:
DAPI Stain
Hematoxylin and Eosin Stain
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HISTOLOGY:
Leica Cryostat (Model CM 1900)
The versatile and uncomplicated use of the Leica CM1900 as well as the reliable results obtained make it the instrument of choice in many routine as well as advanced cryostat applications. With the actively cooled quick freeze shelf (-45°C) up to ten specimens can be frozen rapidly inside the cryochamber, the freezing process being further supported by the built-in heat extractor system. The cryostat can be easily disinfected at a temperature of around -20° C.
Leica Vibrating-blade Microtome (Model VT 1000S)
The Leica VT1000 S is a vibrating blade microtome. The instrument has been designed specifically to meet the highly sophisticated sectioning requirements for special applications in the fields of neurophysiology, neuropathology and experimental (brain) research - patch-clamping technique or ratio imaging tests and some applications related to structural analysis of foam and other very soft materials.
Leica Rotary Microtome (Model RM 2135)
Leica RM 2135 Rotary Microtome with section thickness adjustment from 0-60µm. 0-10µm in 1µm steps, 10-20µm in 2µm steps, 20-60µm in 5µm steps. This microtome is a universal instrument for a variety of applications in laboratories where large specimens embedded in paraffin or hard materials, like methacrylate embedded samples need to be sectioned.
Related Protocols:
Immunohistochemistry (IHC):
IHC- Paraffin Embedded Tissues
Immunofluorescence (IF)
IF-Frozen Tissues
IF-Paraffin Embedded Tissues
IF-Autofluoresence Control
Fluoresence-labeled In situ Hybridization (FISH):
FISH-Cells
Count Staining:
DAPI Stain
Hematoxylin and Eosin Stain
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MOLECULAR ANALYSIS SYSTEMS:
Bio-Rad iCycler Real-Time PCR Detection System
Real-time quantitative PCR provides accurate quantitation. The iCycler iQ allows you to monitor the fluorescent signal of up to 4 different fluorophores in real time. These analyses are implemented as the data are gathered, saving time and effort. iCycler software is designed to automate analysis options, including quantitative analysis, melt curve analysis; and allelic discrimination.
Information:
Quantitative real time PCR Overview
RT-PCR and Realtime PCR
Molecular Beacon Information
SYBR_Green
Powerpoint Slide Show for Realtime PCR
TaqMan-iCycler
Related Protocols:
Total RNA Isolation:
Total RNA From Culture Cells
Total RNA From Tissues
RNA Quality Check
RT-PCR:
Primer Dilutions
RT-PCR Standard Protocol
Real-Time PCR:
Standard Curve
Fluor-S MAX MultiImager
The Fluor-S MAX MultiImager is a quantitative imaging system for capturing digital images from single and multi-color florescence, chemiluminescence, chemifluoresence and colorimetric samples. The Fluro-S MAX MultiImager uses advanced super-cooled CCD technology for image capture. Low temperature cooling of the large, back-thinned CCD improves image quality by reducing background noise and enhancing the signal to noise ratio. The Fluor-S-MAX system incorporates a unique scanning and epi-illumination system for both UV and white light excitation. This provides high-sensitivity imaging of a variety of fluorescent, chemiluminescent and colorimetric samples. The scanning design also provides more uniform illumination than fixed transillumination bulbs whilst still permitting fast image acquisition. The epi- Quantity Oneâ software allows substantial flexibility in the presentation of captured images and provides many tools for data analysis. These include: molecular weight determination, automated lane and band finding, and accurate concentration analysis.
Related Protocol and Information:
Protein Preparation:
Protein Determination:
BCA Protein Assay:
BCA Protein Assay (Pierce Kit)
BCA (in 96 well plate)
BCA (in test tubes)
BCA Protein Assay Reading
DC Protein Assay:
DC Protein Assay (Bio-Rad)
Total Cellular Protein (Bio-Rad)
RC DC Protein Assay Information Sheet (Bio-Rad)
RC DC Protein Instruction Manual (Bio-Rad)
Lowry Protein Assay:
Western Blotting:
Solutions and Reagents
Standard Protocol
Total RNA Isolation:
Total RNA From Culture Cells
Total RNA From Tissues
RNA Quality Check
RT-PCR:
Primer Dilution
RT-PCR Standard Protocol
Real-Time PCR:
Standard Curve
Ribonuclease Protection Assay
Northern Blotting
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Tissue Culture Systems:
CO2 Incubators
Recently, tissue culture core purchased two Thermo water jacked CO2 Incubators (Model 3110 series) which are located at Room #227C HMRC (Tissue Culture Room). One of incubators is specially signed for transfection experiments.
CO2 and O2 control Incubators
Thermo water jacked CO2 and O2 control Incubators (Model 3130 series) is located at Room #625 HMRC. This incubator is specially signed for low oxygen tissue culture experiments.
Desk-top Stean Sterilizer
Recently, tissue culture core purchased Harvey Steam sterilzer (Model Hydroclave MC 10) which is located at Room #227 HMRC. This autoclaver has 10 dia. x 15.5" D chamber and can sterilizes liquids, packs, wrapped and unwrapped materials.
Related Protocol and Information:
Cell Culture:
Seeding
Freezing and Thawing
Cell Viability Assay
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User Fees
Effective December 1, 2002 the PRC's CIHR Group Core Facilities non-member user fees will be charged.
A $25.00/hr fee will be charged for each piece of equipment. The fee will be based on the time that you book the equipment. There is a sign-up sheet and a log-book for each piece of equipment.
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