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  UCSC In-Silico PCR
  
>chrUn.004.7396:4064+4183 120bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtctccagcccagggaaaaaacagagataaaCG
CGACAGGAGAGGAAAtgcCa
>chrUn.004.3593:9982+10097 116bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtctccagcccagggtaaaaacagagataaaCGCGAC
AGGAGAGGAAAtgcCa
>chrUn.004.4066:8601+8728 128bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtgtgtgtctccagcccagggaaaaaacaga
gataaaCGCGACAGGAGAGGAAAtgcCa
>chrUn.004.143:226093+226228 136bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtctccagcccagggaa
aaaacagagataaaCGCGACAGGAGAGGAAAtgcCa
>chrUn.004.415:73161+73282 122bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtctccagcccagggaaaaaacagagataaa
CGCGACAGGAGAGGAAAtgcCa
>chrUn.004.6070:435+554 120bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtctccagcccagggaaaaaacagagataaaCG
CGACAGGAGAGGAAAtgcCa
>chrUn.004.6482:3441+3560 120bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtctccagcccagggaaaaaacagagataaaCG
CGACAGGAGAGGAAAtgcCa
>chrUn.004.1181:26611+26738 128bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtgcgtgtctccagcccagggaaaaaacaga
gataaaCGCGACAGGAGAGGAAAtgcCa
>chrUn.004.7705:1950-2085 136bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtctccagcccagggaa
aaaacagagataaaCGCGACAGGAGAGGAAAtgcCa
>chrUn.004.8315:1651-1782 132bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtctccagcccagggaaaaaa
cagagataaaCGCGACAGGAGAGGAAAtgcCa
>chrUn.004.9533:763-898 136bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtctccagcccagggaa
aaaacagagataaaCGCGACAGGAGAGGAAAtgcCa
>chrUn.004.9614:1650-1773 124bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtgtgtgtctccagcccagggaaaaaacagagata
aaCGCGACAGGAGAGGAAAtgcCa
>chrUn.004.10480:1087-1204 118bp GAGCCTGTGTGTGTATACAC GGCACTTTCCTCTCCTGTCGCG
GAGCCTGTGTGTGTATACACatgtgtgcttgtgtcgcctgtgtgtgtgtg
tgtgtgtgtgtgtgtgtctccagcccagggtaaaaacagagataaaCGCG
ACAGGAGAGGAAAtgcCa

  Primer Melting Temperatures
  Forward: 51.1 C gagcctgtgtgtgtatacac
Reverse: 69.6 C ggcactttcctctcctgtcgcg
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.