Ph.D, Simon Fraser University, 2001
Post-transcriptional regulation of the Cellular Proteome:
Much of our research focuses on cellular processes that regulate the cellular proteome. These include gene silencing by the small miRNAs and protein degradation by the N-End rule pathway.
As our investigations on the biological process of both mi RNAs and protein degradation have been revolving more around mass spectrometry and proteomic applications, our research interests have also begun to include broader proteome wide applications. These include the development of new techniques to applying these techniques to new biological systems.
Fung AWS. Fahlman RP, The molecular basis for the post-translational addition of amino acids by L/F transferase in the N-End rule pathway. Current Prot. Peptide Science. 16:163-180 (2015).
Fung AWS, Leung CCY, Fahlman RP. The Determination of tRNALeu Recognition Nucleotides for Escherichia coli L/F Transferase. RNA 20: 1210-1222 (2014)
Eldeeb M, Fahlman RP. The anti-apoptotic form of Tyrosine Kinase Lyn that is Generated by Proteolysis is Degraded by the N-End Rule Pathway. Oncotarget 5: 2714-2735 (2014).
Chaulk SG, Xu Z, Glover JN and Fahlman RP. MicroRNA miR-92a-1 Biogenesis and mRNA Targeting are Modulated by a Tertiary Contact Within the miR-17~92 Cluster. Nucleic Acids Res. 42: 5234-5244 (2014).
Chaulk SG, Lattanzi VJ, Hiemer SE, Fahlman RP, Varelas X. The Hippo Pathway Effectors TAZ/YAP Regulate Dicer Expression and miRNA Biogenesis Through Let-7. J. Biol. Chem. 289: 1886-1891 (2014).
Fahlman RP, Chen W, and Overall CM. Absolute Proteomic Quantification of the Activity State of Proteases and Proteolytic Cleavages Using Proteolytic Signature Peptides and Isobaric Tags. J. Proteomics 100: 79-91 (2014)
Xu Z, Payoe R and Fahlman RP. The C-terminal proteolytic fragment of the breast cancer susceptibility type I protein (BRCA1) is degraded by the N-end rule pathway. J. Biol. Chem. 287(10):7495-7502 (2012).
Chaulk SG, Thede GL, Kent OA, Xu Z, Gesner EM, Veldhoen RA, Khanna SK, Goping IS, MacMillan AM, Mendell JT, Young HS, Fahlman RP and Glover JNM. Role of pri-miRNA Tertiary Structure in miR-17~92 miRNA Biogenesis. RNA Biology 8(6):1105 - 1114 (2011).