This is a PALM CombiSystem mounted on a Zeiss Axiovert 200M microscope. The setup is used primarily for manipulating cells with laser or selectively picking up cells or sub-cellular structures for biochemical analysis (e.g. single cell PCR). The workstation consists of a pulsed nitrogen laser, which allows precise cutting or micro-dissection. It is also equipped with an infrared laser for trapping and positioning cells.
Example applications for this system:
- Select morphologically identified cells/fragment of cells in pathological sample to study the sample biochemically.
- Using the laser tweezers to physically position 2 cells together to study cell-cell interactions.
- Selective ablation of cells in living tissue or cell culture.
More information about the system can be found on the Zeiss website.