Core Services and Equipment Resources

Electron Microscopes

Hitachi S-4800 Scanning Electron Microscope

1. Resolution:

  • Vacc 15kV at WD 4 mm: 1.0 nm (220,000x)
  • Vacc 1kV at WD 1.5 mm: 2.0 nm (120,000x)

2. Magnification: 

  • High mag mode: 100x to 800,000x
  • Low mag mode: 30x to 2,000x

3. Electron Optics:

  • Electron gun: Cold cathode field emission type
  • Extracting vol (Vext): 0 to 6.5kV
  • Accelerating vol (Vacc): 0.5 to 30kV (in 100V steps)
  • Lens: 3 stage electromagnetic lens, reduction type
  • Obj lens aperture: Movable aperture (4 openings selectable/aligable outside column), Self-cleaning thin aperture
  • Astigmatism correction coil (stigmator): Electromagnetic type
  • Scanning coil: 2 stage electronmagnetic-deflection type 

4. Detection System:

  • Upper SE
  • BSE (High & low angle)
  • Lower SE
  • STEM (Bright & dark field) 

5. Specimen Stage:

  • X traverse: 0 to110 mm (continuous)
  • Y traverse: 0 to 110 mm (continuous)
  • Z traverse: 1.5 to 40 mm (continuous)
  • Tilt: -5 degree to +70 degree
  • Rotation: 360 degree (continuous)
  • Specimen size: Max. 150 mm (diameter) (airlock type specimen exchange) 

6. Evaluation System:

  • System type: Fully automatic pneumatic-valve system
  • Ultimate vacuum levels:
    • Specimen chamber: 7 x 10 to -4 Pa
    • Electron gun chamber:
      • IP1 - 1 x 10 to -7 Pa or better
      • IP2 - 2 x 10 to -6 Pa or better
      • IP3 - 7 x 10 to -5 Pa or better
  • Vacuum pumps:
    • Electron optical system: 3 ion pumps
    • Specimen chamber: Turbomolecular pump 
  • Compressor: Oil-less type compressor 

7. Protection Devices:

  • Warning devices: Power failure, cooling-water interruption, inadequate vacuum

Hitachi H-7650 Transmission Electron Microscope

This transmission electron microscope (TEM) can achieve 70X to 200,000X magnification with 40 to 120 kv accelerating voltage. The TEM also can achieve maximum of 500,000X magnification in the high-resolution mode. The 16 megapixel EMCCD camera mounted at the low-mount position (Advanced Microscopy Techniques) generates high quality images of ultrastructure.