Core Services and Equipment Resources

Core Personnel

Featured Biography

Sabina Baghirova
Technologist, Flow Cytometry Facility

 Sabina Baghirova

Sabina Baghirova is the Technologist of the Flow Cytometry Facility. She obtained her BSc in Biochemistry and Biotechnology from Carleton University, and MSc in Pharmacology from the University of Alberta in the lab of Dr. Richard Schulz. Her thesis focused on nuclear localization of Matrix Metalloproteinase-2 and investigation of its potential targets in myocardial ischemia-reperfusion injury. This work included using imaging flow cytometry to show nuclear localization of Matrix Metalloproteinase-2. Shortly after obtaining her MSc in 2016, she joined the Flow Cytometry Facility, where she provides assistance with cell sorting, performs daily maintenance on all the equipment and provides training on both analyzers and sorters.

The Flow Cytometry Facility provides services in experimental design, analytical flow cytometry, cell sorting, and data analysis. The core currently has nine instruments divided between the main facility in HMRC, and satellite facilities in Katz. The instruments include a wide range of analyzers (FACSCanto II, LSR-Fortessa, and Attune NxT), sorters (BD FACS ARIA III) and an imaging flow cytometer (Amnis Image stream MkII).

You may contact Sabina to find out how to get started in utilizing the services offered at the Flow Cytometry Facility.

Contact Information:
E-mail: baghirov@ualberta.ca
Phone: 780-492-7780
Office/Facility: 6-67 HMRC

 

Previous Core Staff Biographies 

Woo Jung Cho
Senior Electron Microscopy Lead, Cell Imaging Centre

 Woo Jung Cho

Woo Jung Cho is the Senior Electron Microscopy Lead of the FoMD Cell Imaging Centre. He obtained his BSc in Biology (Soonchunhyang University, 1996) and MSc in Zoology (Chung-Ang University, 1998) in South Korea. His MSc research focused on a morphometric study on the distribution of neurotransmitters in the myenteric and submucous plexuses of the small intestine of the rat using whole-mount immunohistochemistry, light microscopy and transmission electron microscopy. During his MSc, he taught an Histology Lab at Konkuk University School of Medicine. Following his MSc, Woo Jung worked at Samsung Medical Centre as a medical science researcher and taught a Human Anatomy Lab at Ajou University School of Medicine as a teaching assistant in South Korea. In 2002, he started working with Dr. Edwin Daniel (Pharmacology, University of Alberta) as a research technologist researching gastrointestinal smooth muscle cells. In 2009 he was appointed chief electron microscopy technician in the Department of Cell Biology's Electron Microscopy Facility at the University of Alberta. In 2011 he moved to Princeton University to run the Electron Microscopy & Histology Core Facility (Department of Molecular Biology) as a manager. In 2012 he returned to the University of Alberta to join the FoMD Cell Imaging Centre. 

The Cell Imaging Centre - Electron Microscopy (EM) Facility is located in B-120 Katz Group Centre and maintains one high-resolution transmission electron microscope (120 kV TEM), one high-resolution field emission gun scanning electron microscope (FEG-SEM), and sample preparation equipment. This facility provides fee-based training in both sample preparation and EM equipment operation for standard EM projects (conventional TEM/SEM and immuno-TEM/SEM). The EM Facility also offers fee-based services for advanced EM projects (cryo TEM, cryo immuno-TEM, serial section SEM and correlative light & electron microscopy). The EM Facility continues to develop protocols and services for University of Alberta researchers based on recent advances in EM. 

Contact information:
E-mail: woojung.cho@ualberta.ca
Phone: 780-492-9086
Office: B-120 Katz Group Centre

 

Peter Burns
Workshop Technician, Workshop

Peter Burns

Peter Burns is the Workshop Technician in the Faculty of Medicine & Dentistry (FoMD) Workshop. He obtained his Electronics and Engineering Technology Diploma with Honors from NAIT in 2014 and a Bachelor of Engineering Technologies: Electronics and Controls from Cape Breton University. Before joining the University of Alberta, he was an Electro-Optics Technician with Alberta Nano-Monitoring Systems. Since joining the U of A in 2016, he has expanded his skill set in understanding the mechanical side of equipment and recently began an apprenticeship in machining. 

The Workshop provides a number of services, including repair of lab equipment and biosafety cabinets, metal and acrylic fabrication, woodworking, welding, and machining.

Contact information:
E-mail: pburns@ualberta.ca
Phone: 780-492-6526
Office/Facility:7-58 HMRC

Greg Plummer
Coordinator, Cell Imaging Core Facility

  

Greg Plummer is the Coordinator of the Cellular Imaging Core Facility. He obtained a B.Sc. with Specialization, and a M.Sc. in Pharmacology from the University of Alberta in the lab of Dr. Elena Posse de Chaves. His thesis focused on novel survival effects of sphingolipids in apoptotic neuronal cultures. He has worked extensively as a research assistant in academic labs (Cell Biology, University of Alberta) and in academic-biotech partnerships (University of Dundee, Scotland and Servier Inc.) In 2008 he returned to Edmonton to work in the Alberta Diabetes Institute (ADI) under Dr. Patrick Macdonald and in 2010 was appointed the coordinator of the ADI Imaging Core. In 2014 the ADI Imaging Core merged with the Faculty of Medicine & Dentistry (FoMD) Cellular Imaging Core to create a dynamic large scale imaging facility to serve the entire University of Alberta as well as outside partnerships.

The Cellular Imaging Core Facility offers both light and electron microscopy. Greg administers all of the fluorescent and live-cell imaging in the facility. The Cellular Imaging Core contains widefield, live-cell, confocal, laser dissection and structured illumination super resolution fluorescence microscopy. The facility also provides consultation on experimental design and analysis of microscope images. The main facility is located in the basement of the Katz Group Centre with satellite facilities in the adjoining HMRC and LKS buildings. The Cellular Imaging Core Facility currently receives support from FoMD and the Canada Foundation for Innovation. 

Contact information:
E-mail: gplummer@ualberta.ca
Phone: 780-492-2181
Office/Facility:B-120 Katz Group Centre

 

Sam Graziano
Supervisor, Workshop

 Sam Graziano 

Sam Graziano is the Supervisor of the Faculty of Medicine & Dentistry (FoMD) Workshop. He started working in the Pharmacology/Physiology Workshop as a technician in 1985 under the supervision of electronics technologist Nick Diakiw. In the late 1980s and early 1990s, Sam took several classes at NAIT to better hone his skills, including those in machining, welding, carpentry, electronics and small animal handling. The Workshop moved to its current location in the Heritage Medical Research Centre in 1995 and began providing services to researchers in FoMD and across the University of Alberta. Sam became Supervisor of the Workshop in 1999 and received his Journeyman Machining ticket in 2001.

The Workshop provides a number of services, including repair of lab equipment and biosafety cabinets, metal and acrylic fabrication, woodworking, welding, and machining.

Contact information:
E-mail: graziano@ualberta.ca
Phone: 780-492-6526
Office: 7-58 HMRC

 

Dr. Aja Rieger
Manager, Flow Cytometry Facility

 Aja Rieger

 Dr. Aja Rieger is the manager of the Flow Cytometry Facility. She obtained her BSc in Immunology and Infection (University of Alberta), MSc in Microbiology and Immunology (McGill University), and PhD in Physiology, Cell, and Developmental Biology (University of Alberta). Her PhD research focused on mechanisms of inflammatory control by phagocytes. During her PhD, she also developed multiple assays and protocols to study inflammation in non-classical animal models, primarily using imaging flow cytometry. Following her PhD, Aja pursued post-doctoral studies at University of California - Berkeley. She then joined the Flow Cytometry Facility in 2015, were she continues to develop flow cytometry assays with researchers on campus. Aja is also an 'International Society for the Advancement of Cytometry Shared Resource Lab Emerging Leader (2017-2021)', the highest international award for Flow Cytometry Core Facility managers.

Services in the Flow Cytometry Facility have existed on campus since 1981. The Flow Cytometry Facility offers both fee-for-service sample analysis and sorting, as well as full training services for both the analyzers and sorters. We also offer a comprehensive educational program, with an Introductory course offered 3 times a year and monthly workshops focused on selected topics. The Flow Core currently offers instruments capable of analyzing both BSL1 and BSL2 samples, with cytometers configured for analysis of up to 18 fluorochromes.

Contact information:
E-mail: aja@ualberta.ca
Phone: 780-492-6721
Office: 6-020C Katz Group Centre

 

Dr. Serene Wohlgemuth
Manager, Transgenic Core Facility

 

Dr. Serene Wohlgemuth is the manager of the Transgenic Core Facility. She obtained her BSc in Molecular Genetics and her PhD in Molecular Biology and Genetics, both from the University of Alberta. Her PhD focused on developmental genetics in zebrafish embryos, specifically the role of a chaperone-like protein in muscle and heart development. Following her PhD, she began work as a research technologist in the Centre for Prions and Protein Folding Diseases (CPPFD). This work, with a focus on molecular biology, was under Dr. David Westaway. Building upon her experience in embryo injection and with initial funding from the PrioNet NCE and the CFI to Dr. Westaway, she commenced the production of transgenic mice by pronuclear microinjection. Working in the CPPFD facility, since 2010 she has since made 11 new transgenic lines for researchers at the University of Alberta and elsewhere. 

Central transgenic services at the University of Alberta were disbanded with departure of Dr. Peter Dickie in 2007 and were re-established as a core facility run by Dr. Wohlgemuth in 2012. This core facility offers services related to the production of traditional transgenic mice through pronuclear microinjection and, more recently, has initiated experiments on CRISPR-Cas9 genetic engineering. It also provides mouse sperm cryopreservation and sperm and embryo cryo-recovery. It recently moved into a new space in the HSLAS facility and will soon be able to provide re-derivation services for the production of pathogen-free mouse stocks. 

Contact information:
E-mail: slw1@ualberta.ca
Phone: 780-492-9303

 

Mr. Audric Moses
Coordinator, Lipidomics Core Facility

Audric Moses

Audric Moses is the Coordinator of the Lipidomics Core Facility. He obtained a B.Sc. with Specialization in Microbiology and a Minor in Immunology with Distinction, from the University of Alberta. He then went on to complete a M.Sc. in Experimental Medicine from the U of A in the lab of Dr. Gordon Francis (Department of Medicine, Division of Endocrinology). His thesis focused on the oxidation of high density lipoproteins (HDL) and cholesterol movement through the artery wall. He was then recruited by Dr. Dennis Vance (Department of Biochemistry) of the CIHR Group on the Molecular and Cell Biology of Lipids (MCBL) as a group Research Assistant performing analytical measurements of lipids and lipoproteins, overseeing all major and minor equipment, and acting as local network administrator. When CIHR discontinued this program, the MCBL spun off the Lipidomics Core Facility and Mr. Moses was hired under Dr. Richard Lehner (Department of Pediatrics) as the sole employee of the facility. This restructuring was made possible by major support from the Women and Children & Health Research Institute (WCHRI).

The Lipidomics Core Facility offers routine analysis of lipids and lipoproteins by HPLC, GC, and FPLC, with ongoing development of orbitrap mass spectrometry services, as well as custom HPLC and GC method development and analysis as needed. The lab is located in the FoMD mass spectrometry hub in the Katz Group Centre. In addition to providing services to researchers in the faculty and at the University of Alberta, the facility regularly analyzes samples from other North American research institutions, and as far away as Austria and Japan. The Lipidomics Core Facility currently receives support from the Faculty of Medicine & Dentistry and WCHRI.

Contact information:
E-mail: audric.moses@ualberta.ca
Lab: 4-096 Katz Group Centre

 

Dr. Juan Jovel
Manager and Bioinformatics Lead, The Applied Genomics Core

Juan Jovel 

Dr. Juan Jovel is the new manager of The Applied Genomics Core (TAGC) and Bioinformatics lead. Dr. Jovel obtained his PhD in Cellular and Molecular Biology from the Institute of Biomaterials and Biomolecular Systems at the University of Stuttgart in Germany. He then worked on numerous research projects in several institutions, including Agriculture Canada (Summerland, British Columbia), The Scripps Research Institute (La Jolla, CA), The University of California Riverside, and more recently, as a member of the group of Professor Gane Wong in the Department of Medicine of the University of Alberta. During the last eight years, Dr. Jovel has focused on bioinformatics analysis of data derived from diverse research and clinical projects pertaining to the areas of genomics, transcriptomics and metagenomics.

TAGC offers a variety of molecular biology services, including Sanger sequencing, real time PCR, animal genotyping, quantification of nucleic acids using Qubit and Bioanalyzer instruments, and Next Generation Sequencing (NGS) services, including library preparation, MiSeq (and soon Ion S5) sequencing and bioinformatics analysis.

Dr. Jovel welcomes you to use the services available at TAGC and to contact him to discuss how NGS and bioinformatics approaches can leverage your research program.

Contact information:
E-mail: jovel@ualberta.ca
Phone: 780-492 9520
Office: 7-12 HMRC


Dr. Aja Rieger is the manager of the Flow Cytometry Facility. She obtained her BSc in Immunology and Infection (University of Alberta), MSc in Microbiology and Immunology (McGill University), and PhD in Physiology, Cell, and Developmental Biology (University of Alberta). Her PhD research focused on mechanisms of inflammatory control by phagocytes. During her PhD, she also developed multiple assays and protocols to study inflammation in non-classical animal models, primarily using imaging flow cytometry. Following her PhD, Aja pursued post-doctoral studies at University of California- Berkeley. She then joined the Flow Cytometry Facility in 2015, were she continues to develop flow cytometry assays with researchers on campus. Aja is also an 'International Society for the Advancement of Cytometry Shared Resource Lab Emerging Leader (2017-2021)', the highest international award for Flow Cytometry Core Facility managers.
 
Services in the Flow Cytometry Facility have existed on campus since 1981. The Flow Cytometry Facility offers both fee-for-service sample analysis and sorting, as well as full training services for both the analyzers and sorters. We also offer a comprehensive educational program, with an Introductory course offered 3 times a year and monthly workshops focused on selected topics. The Flow Core currently offers instruments capable of analyzing both BSL1 and BSL2 samples, with cytometers configured for analysis of up to 18 fluorochromes. 
Dr. Aja Rieger is the manager of the Flow Cytometry Facility. She obtained her BSc in Immunology and Infection (University of Alberta), MSc in Microbiology and Immunology (McGill University), and PhD in Physiology, Cell, and Developmental Biology (University of Alberta). Her PhD research focused on mechanisms of inflammatory control by phagocytes. During her PhD, she also developed multiple assays and protocols to study inflammation in non-classical animal models, primarily using imaging flow cytometry. Following her PhD, Aja pursued post-doctoral studies at University of California- Berkeley. She then joined the Flow Cytometry Facility in 2015, were she continues to develop flow cytometry assays with researchers on campus. Aja is also an 'International Society for the Advancement of Cytometry Shared Resource Lab Emerging Leader (2017-2021)', the highest international award for Flow Cytometry Core Facility managers.
 
Services in the Flow Cytometry Facility have existed on campus since 1981. The Flow Cytometry Facility offers both fee-for-service sample analysis and sorting, as well as full training services for both the analyzers and sorters. We also offer a comprehensive educational program, with an Introductory course offered 3 times a year and monthly workshops focused on selected topics. The Flow Core currently offers instruments capable of analyzing both BSL1 and BSL2 samples, with cytometers configured for analysis of up to 18 fluorochromes. 
Dr. Aja Rieger is the manager of the Flow Cytometry Facility. She obtained her BSc in Immunology and Infection (University of Alberta), MSc in Microbiology and Immunology (McGill University), and PhD in Physiology, Cell, and Developmental Biology (University of Alberta). Her PhD research focused on mechanisms of inflammatory control by phagocytes. During her PhD, she also developed multiple assays and protocols to study inflammation in non-classical animal models, primarily using imaging flow cytometry. Following her PhD, Aja pursued post-doctoral studies at University of California- Berkeley. She then joined the Flow Cytometry Facility in 2015, were she continues to develop flow cytometry assays with researchers on campus. Aja is also an 'International Society for the Advancement of Cytometry Shared Resource Lab Emerging Leader (2017-2021)', the highest international award for Flow Cytometry Core Facility managers.
 
Services in the Flow Cytometry Facility have existed on campus since 1981. The Flow Cytometry Facility offers both fee-for-service sample analysis and sorting, as well as full training services for both the analyzers and sorters. We also offer a comprehensive educational program, with an Introductory course offered 3 times a year and monthly workshops focused on selected topics. The Flow Core currently offers instruments capable of analyzing both BSL1 and BSL2 samples, with cytometers configured for analysis of up to 18 fluorochromes.