Core Services and Equipment Resources

Other Molecular Biology Techniques

Sanger Sequencing

TAGC houses a 3130xl (16-capillary) Life Technologies Genetic Analyzer and has the capacity to process 192 samples per day. We use BigDye Terminator v3.1 Cycle Sequencing Kit and Axygen CleanSEQ, a magnetic beads-based post-reaction clean up protocol, automated on a Biomek 3000 work station. We also offer assistance with data analysis and protocol troubleshooting.

Sanger Sequencing runs every Tuesday, Thursday, and Friday. Please drop off samples and submit the submission form via iLab by 3:30PM the day before sequencing (Monday, Wednesday, or Thursday) in 8-strip PCR tubes (available from TAGC at no additional cost). Primers are available for purchase from TAGC; please log into iLab for additional details.

Sanger sequencing services cost $4.75/sample for all researchers within the University of Alberta and $6.00/sample for all external users.

Genotyping

TAGC offers customized protocols for DNA extraction and PCR amplification from mouse and rat tissues (e.g. ear and tail) using the KAPA Mouse Genotyping Kit. The PCR products are examined on the QIAxcel, a fully automated capillary electrophoresis system, which reports both gel images and electropherograms, and determines the size and concentration of DNA fragments.

Nucleic Acids Extraction and Evaluation

TAGC offers standard DNA and RNA extraction services at a cost of $12/sample. Other nucleic acid extraction services are available; please contact us for details.

Determination of the size, concentration and quality of DNA and RNA is performed on automated electrophoresis instruments, which significantly increase the number of samples that can be processed at a time and produce a normalized digital output that can be used for comparisons within a run or between various runs. TAGC currently has two automated DNA/RNA Electrophoresis instruments and one dye-based nucleic acid quantification instrument:

Qiagen QIAxcel
Agilent Bioanalyzer 2100
Qubit Fluorometer

These instruments are available to be run by TAGC technicians as a service at a rate of $80/hr + reagent costs.

Real-Time PCR

TAGC houses a 7900HT Fast Real-Time PCR System, with 3 interchangeable blocks, for:

96-well fast plates
96-well standard plates
384-well fast plates

The type of assays that can be performed on the 7900HT are:

  • Allelic discrimination (quantitative analysis)
  • Relative quantification of gene expression
  • Absolute quantification
  • Dissociation curve

The 7900HT supports both Taqman and SYBR green assays.

TAGC provides training for $80. Upon completion of this training users can book and run the instrument during TAGC hours at a cost of $20/run.