Core Services and Equipment Resources

Fee Schedule

Service Description Fee
Sperm Cryopreservation User will supply one proven breeder male (ideally <6 months old) to the facility. Sperm will be harvested and approximately nine (9) aliquots of sample frozen. A basic motility check will be performed for quality assessment.

$300

Sperm Storage Annual fee to store samples under liquid nitrogen. Aliquots of each sample will be divided among two tanks.

$100

in vitro Fertilization Unfertilized oocytes collected from five (5) FVB* females and mixed with sperm. Fertilized oocytes transferred into recipients and pups gestated until weaned. Tissue sample provided for genotyping.

$,2100

Pronuclear Injection
(Transgenic Mouse Production)
Three (3) rounds of injection, collecting from eight (8) mice each round. Approximately 300 embryos collected. Surviving embryos transferred into recipients and pups gestated until weaned. Tissue sample provided for genotyping.
$4,800
CRISPR/Cas9 
Three (3) rounds of injection and/or electroporation, collecting from eight (8) mice each round. Approximately 300 embryos collected. Surviving embryos transferred into recipients and pups gestated until weaned. Tissue sample provided for genotyping. NOTE: All CRISPR/Cas9 reagents to be provided by user.
$4,200
 Rederivation Unfertilized oocytes from five commercially-available females are collected and mixed with sperm from the male of the "contaminated" stain. Embryos are thoroughly washed and transferred into pathogen-free recipients. Pups will remain with the recipient female until weaning. Following weaning, Mouse Assessment Plus serology of the dams and PCR Infectious Agent (PRIA) testing of both the dams and pups will be completed by Charles River Laboratories.

 $3,000

*Additional fees may occur if extended housing or additional testing required.