Sequence Manipulation Suite:
PCR Products
PCR Products accepts one or more DNA sequence templates and two primer sequences. The program searches for perfectly matching primer annealing sites that can generate a PCR product. Any resulting products are sorted by size, and they are given a title specifying their length, their position in the original sequence, and the primers that produced them. You can use linear or circular molecules as the template. Use PCR Products to determine the product sizes you can expect to see when you perform PCR in the lab.

Paste the raw sequence or one or more FASTA sequences into the text area below. Input limit is 200000 characters.

Enter the name of the first primer, followed by its sequence in the 5' to 3' direction. Degenerate sites can be represented using IUPAC DNA characters.

Enter the name of the second primer, followed by its sequence in the 5' to 3' direction. Degenerate sites can be represented using IUPAC DNA characters.

Please check the browser compatibility page before using this program.
  • Treat sequences as molecules.
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Sun Oct 28 03:52:59 2012
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