The BME Seminar Series features presentations surrounding the field of Biomedical Engineering.
Next Upcoming Seminar:
Date: 2018 October 23
Time: Noon - 1PM
Location: 1-075 RTF
Presentation Topic: Clinical Utility of Proposed Gait Stability Measures: Selection, Application and Evaluation of the Extrapolated Centre of Mass
Presenter: Jeremy Hall, MSc. candidate
In humans, gait stability is a complicated, multifaceted process which makes it difficult to establish an exact definition. This has led to the development of a variety of proposed stability measures, each with different characteristics affecting their practical application. In light of these considerations, the objectives were to: (1) select the measures that are clinically feasible; (2) demonstrate the practical feasibility of obtaining the selected measures and assess their robustness for a non-disabled sample; (3) evaluate the between-session reliability; and (4) demonstrate the clinical utility using three clinical case studies. From the selection process, the extrapolated centre of mass (XCoM) best demonstrated the characteristics of clinical feasibility. To quantify stability, the XCoM must remain within the limits of the base of support. The degree of stability is then given by the margin of stability (MoS): the minimum distance between the XCoM and the base of support. Non-disabled participants were asked to walk in the Computer-Assisted Rehabilitation Environment on a treadmill-driven surface. All participants returned for a repeatsession several weeks following initial testing. Three case study participants with hemophilic arthropathy, unilateral transtibial amputation, and mild traumatic brain injury were also included, each completing one session. Results suggest that the MoS at heel strike (MoS-HS) is best suited to quantifying stability in both the mediolateral and anteroposterior directions. Not only does MoSHS demonstrate reasonable variability, it also showed good repeatability between sessions. Furthermore, MoS-HS provided promising results towards highlighting differences between body sides in the case study participants.
All are welcome!
The BME Seminar Series features presentations by current graduate students as part of the BME 600A/B courses (Fall/Winter terms). Each of the seminars offers a glimpse into biomedical research taking place at our university.
Details about the next series will be posted soon!
Title: Exploring RNA Interference as a Therapeutic Option for Acute Myeloid Leukemia using Lipopolymer Delivery Systems
Speaker: Anyeld Ubeda
Date: Wednesday, Nov. 7, 2018
Time: Noon to 1:00 PM
Venue: ECHA L1-220
Acute myeloid leukemia (AML) is the most prevalent myeloid disorder in adults with high mortality rates due to patient relapse. AML originates in the hematopoietic stem and progenitor cells and is characterized by the increase of immature myeloblasts or “blasts” in circulation overcrowding healthy cells and space in the bone marrow. In this project, we focused on gene silencing via RNA interference (RNAi) with silencing RNA (siRNA) as a targeted therapeutic approach, able to be directed to specific genetic mutations or genes that get over-expressed in leukemia cells compared to healthy tissues. As the specificity of RNAi therapy is given by the siRNA sequence, using this type of approach can offer alternatives and potent combinations to treat AML other than relying on protein inhibitors or chemotherapeutic agents with broad mechanisms of action. However, even though the use of siRNAs has great potential for therapeutic applications some obstacles prevent it from being readily used, mainly that it needs a delivery system.
Given their ease of synthesis and chemical flexibility with the incorporation of countless functional groups, we used modified lipopolymers to address siRNA delivery. More specifically, we used low molecular weight polyethyleneimine (PEI) modified with aliphatic groups, which have shown to make PEI into an effective delivery agent for AML cells in the past. By analyzing a library of modifications, we identified two main substitutions that were able to consistently and effectively transfect leukemic stem cell models and primary samples. Next, we utilized the modified lipopolymers to downregulate the expression of genes that target the proliferation and apoptosis of the leukemic cells and focused on the BCL2 Like 12 (BCL2L12) and Baculoviral IAP Repeat Containing 5 (BIRC5 or survivin) genes. Also, by optimizing the siRNA delivery schedule we were able to enhance the effect of the siRNAs on proliferation over a period of 10 days. In addition, we showed how after selecting the proper modifications for PEI we are also able to target multiple genes including MAP2K3, CDC 20, and SOD-1 and observed decreased proliferation in the desired cells. Our studies demonstrated the versatility of siRNA delivery with modified PEI to elicit an effect in leukemic cells that are difficult to treat as well as paramount therapeutic targets for AML, offering an alternative for more precise and targeted treatment options.
RTF Table Tennis Tourney April 2018!
Want to play table tennis (ping-pong)?
Want to meet other people from RTF/Imaging Research?
Why not kill 2 birds with 1 stone?
If you're interested, e-mail Prayash and Kevin (or let them know in person)
Update 4 April 2018:
The paddles are here, the table is set, and the nets are waiting!
Tentatively, we are planning the tournament day during the week of April 23-27, but please use our Table Tennis Tourney Doodle Poll to indicate which day works best for you. The tourney bracket will include everyone who signs up.
We will also have a practice session some time prior to the tournament. We will use this to go over the rules, wipe some of the rust off, and make sure everyone is on the same page! Everyone who is interested can fill out the Practice Day Doodle Poll.
Keep a look out for an e-mail next Friday (April 13) confirming the date and time!
Please feel free to forward this to anyone in BME who may not have received it.
Prayash - room 1-086 RTF
Kevin - room 1-113 RTF