MolBioCore
The MolBioCore offers a comprehensive array of equipment and provides technical consultation and support for projects such as DNA cloning to make expression vectors, real-time PCR analysis, recombinant protein production and protein purification. Technical training can also be provided for self-directed equipment use.
- Designing of DNA construct and DNA cloning to create expression vectors
- Preparation of plasmid DNA
- Electrophoresis of protein and DNA samples
- Western blot analysis
- Protein/peptide purification using FPLC/ HPLC
- Production of recombinant protein
- Quantification of gene expression by real-time PCR
- Designing and development of gene specific real-time PCR
- Quantification of hormones and cytokines by immunoassays
Technical training $65/hr
Highly qualified staff provides technical assistance on procedures and method development related to molecular biology and protein chemistry
Unassisted Instrumentation Use
MBioCore uses automated, high quality instruments to produce efficient and reliable results.
Centrifugation
| Avanti J-26 XP/XP1 High performance | $7 / hour |
| J6 MI High Capacity | $7 / hour |
| Optima L-100XP Ultracentrifuge | $14 /hour |
Plate Readers
| EnVision 2104 | $6 / plate |
| MSD SECTOR Imager 6000 multiplex | $11 / plate |
Plate Washer
| Skan Washer 400 | $3 / use |
PCR
| Applied Biosystems 7900HT Realtime | $14 / use |
| BioRad T-100 Thermal Cycler | $ 4 / use |
DNA/RNA/Protein Quantification
| Nanodrop 1000/3000 | $2 / use |
Imaging
| GE ImageQuant | $2 / photo |
| GE Typhoon Trio | $10 / scan |
| GE InCell Analyzer 6000 | $20 / plate |
| Olympus OV110 Small animal imager | $55 / hour |
Alberta Diabetes Institute members receive 25% off price list
Equipment Access and Use
Monday-Friday from 9.00-18.00
- Mandatory training for all first-time users
- to book an appointment kunimasa@ualberta.ca Email Kuni Suzuki (Core Manager)
- Security card access available for users
- Instruments must remain free of debris, spills or damage following use
Note: Cleaning/repair costs will be charged back to user
Molecular biology protocols are diverse and experience counts when it comes to developing protocols that bring you results with high quality. MolBioCore has extensive experience optimizing protocols and providing assistance on its equipment.
MOLBIOCORE
Alberta Diabetes Institute
6-030 Li Ka Shing Centre
University of Alberta
Core Manager - Kuni Suzuki kunimasa@ualberta.ca
Core Director - John Elliott