Hwang Biomolecular NMR Group

Our Lab

Our lab is situated in the Department of Biochemistry at the University of Alberta. We are interested in characterizing life processes at the atomic level using multi-nuclear (non-radioactive ¹H, ¹³C, and ¹⁵N) multi-dimensional solution NMR spectroscopy, along with other biophysical techniques. We have a number of ongoing projects, ranging from the development of solution NMR methodology to protein structure determination to clinical application.

Our Lab Publications

• Zahran, S., Figueiredo, V. P., Graham, M. M., Schulz, R. & Hwang, P. M. Proteolytic Digestion of Serum Cardiac Troponin I as Marker of Ischemic Severity. The Journal of Applied Laboratory Medicine jalm.2017.025254 (2018). doi:10.1373/jalm.2017.025254 [ahead of print]
• Mahmud, Z. & Hwang, P. M. Cardiac Troponin Complex: Cardiac Troponin C (TNNC1), Cardiac Troponin I (TNNI3), and Cardiac Troponin T (TNNT2). Encyclopedia of Signaling Molecules. (2017)
• Danmaliki, G. I., Liu, P. B. & Hwang, P. M. Stereoselective Deuteration in Aspartate, Asparagine, Lysine, and Methionine Amino Acid Residues Using Fumarate as a Carbon Source for Escherichia coli in D2O. Biochemistry 56, 6015-6029 (2017).
• Cai, F., Li, M.X., Pineda-Sanabria, S.E., Gelozia, S., Lindert, S., West, F., Sykes, B.D., and Hwang, P.M. Structures reveal details of small molecule binding to cardiac troponin. J. Mol. Cell. Cardiol. 101, 134-144 (2016).
• Hwang, P.M. "(De-)sensitization" vs. "Uncoupling": what drives cardiomyopathies in the thin filament? Cardiovasc. Res. 109, 185-186. (2016).
• Li, M. X. & Hwang, P. M. Structure and function of cardiac troponin C (TNNC1): Implications for heart failure, cardiomyopathies, and troponin modulating drugs. Gene 571, 153-166 (2015).
• Hwang, P. M. & Sykes, B. D. Targeting the sarcomere to correct muscle function. Nat Rev Drug Discov 14, 313-328 (2015).
• Zahran, S., Pan, J. S., Liu, P. B. & Hwang, P. M. Combining a PagP fusion protein system with nickel ion-catalyzed cleavage to produce intrinsically disordered proteins in E. coli. Protein Expr. Purif. 116, 133-138 (2015).

Lab Members