Flow Cytometry Facility Personnel

Aja Rieger

Aja Rieger
Manager, Flow Cytometry Facility

Dr. Aja Rieger is the manager of the Flow Cytometry Facility. She obtained her BSc in Immunology and Infection (University of Alberta), MSc in Microbiology and Immunology (McGill University), and PhD in Physiology, Cell, and Developmental Biology (University of Alberta). Her PhD research focused on mechanisms of inflammatory control by phagocytes. During her PhD, she also developed multiple assays and protocols to study inflammation in non-classical animal models, primarily using imaging flow cytometry. Following her PhD, Aja pursued post-doctoral studies at University of California - Berkeley. She then joined the Flow Cytometry Facility in 2015, were she continues to develop flow cytometry assays with researchers on campus. Aja is also an 'International Society for the Advancement of Cytometry Shared Resource Lab Emerging Leader (2017-2021)', the highest international award for Flow Cytometry Core Facility managers.

Services in the Flow Cytometry Facility have existed on campus since 1981. The Flow Cytometry Facility offers both fee-for-service sample analysis and sorting, as well as full training services for both the analyzers and sorters. We also offer a comprehensive educational program, with an Introductory course offered 3 times a year and monthly workshops focused on selected topics. The Flow Core currently offers instruments capable of analyzing both BSL1 and BSL2 samples, with cytometers configured for analysis of up to 18 fluorochromes.


Sabina Baghirova

Sabina Baghirova
Technologist, Flow Cytometry Facility

Sabina Baghirova is the Technologist of the Flow Cytometry Facility. She obtained her BSc in Biochemistry and Biotechnology from Carleton University, and MSc in Pharmacology from the University of Alberta in the lab of Dr. Richard Schulz. Her thesis focused on nuclear localization of Matrix Metalloproteinase-2 and investigation of its potential targets in myocardial ischemia-reperfusion injury. This work included using imaging flow cytometry to show nuclear localization of Matrix Metalloproteinase-2. Shortly after obtaining her MSc in 2016, she joined the Flow Cytometry Facility, where she provides assistance with cell sorting, performs daily maintenance on all the equipment and provides training on both analyzers and sorters.

The Flow Cytometry Facility provides services in experimental design, analytical flow cytometry, cell sorting, and data analysis. The core currently has nine instruments divided between the main facility in HMRC, and satellite facilities in Katz. The instruments include a wide range of analyzers (FACSCanto II, LSR-Fortessa, and Attune NxT), sorters (BD FACS ARIA III) and an imaging flow cytometer (Amnis Image stream MkII).

You may contact Sabina to find out how to get started in utilizing the services offered at the Flow Cytometry Facility.