Core Services and Equipment Resources

Instruments

Cell Sorters

Most high speed cells sorters (e.g BD FACS Arias or the MoFlow) make use of the electrostatic deflection of droplets method. Fluid containing particles passes through a nozzle (between 50 and 100 µm diameter) and the stream emerges into the air. The fluid stream breaks up into droplets- with the aim being the generation of droplets big enough to contain one cell in each drop. To do this, the stream is the vibrated at a specific frequency and pressure (usually between 20 and 60 kHz kHz)  to separate it into droplets by a piezoelectric transducer. These droplets are then charged (± v where v typically lies between 50 and 150 V) or not depending upon the fluorescent profile of the cell(s) within. The drops pass through an electric field allowing electrostatic or droplet deflection which sends the charged drops of interest into microcentrifuge tubes, 6-mL centrifuge tubes, 15- or 50-mL conical tubes, multiwell plates (anything up to 384-well plates) or onto a slide. Unsorted droplets go into the waste.

Click on the name of the device to learn more:

Aria III BD Aria III  BSL2
   


Cell Analyzers

Our Flow Cytometry analysers are based on differential pressure and are designed to analyze cells at a high rate (up to ca 10,000 – 40,000 cells (events) per second on the perfect single cell sample).Several parameters can be measured on individual cells within a few minutes. This is the power of flow cytometry. The properties measured include a particle’s relative size, granularity, and relative fluorescence intensity (please see the individual machines for the number of fluorochromes they can analysis in one cell/particle). Any suspended particle or cell from 0.2-70 micrometers in size is suitable for analysis. Larger or smaller particles require specialized cytometers/conditions.

Click on the name of the device to learn more:

Attune NxT
BD FACSCanto II BD Fortessa SORP
Amnis ImageStream
BD Fortessa
BD Fortessa X-20

 


Instrument descriptions

Cell sorters

Aria III | Instrument Configuration

The BD FACSAria III cell sorter is the high-speed  benchtop digital flow cytometer. It is equipped with five spatially separated air-cooled lasers – 488 nm, 633 nm, 561 nm,375 nm and 405 nm. The near UV (375 nm) laser use the same path as violet (405 nm) laser 375nm and cannot be use simultaneously.

Sorting can be performed into two or four tubes, at three flow rates, with the speed of up to 35,000 events/sec. Most cell types can be accommodated with the use of 100, 85 or 70 micron nozzles.  The Automated Cell Deposition Unit (ACDU) makes it possible to sort individual cells into multi-well plates, PCR tubes or microscope slides. Instrument is also equipped with Aerosol Management Option (AMO), a device that traps aerosolized particles. The system operates on a PC-based Windows XP platform using BD FACSDiva software.

Location: Room 667 Heritage Medical Research Centre (HMRC)

BD Aria III  BSL2  (Katz 6-125) (16-color) | Instrument Configuration

The BD FACSAria III cell sorter is the high-speed  benchtop digital flow cytometer. It is equipped with five spatially separated air-cooled lasers – 488 nm, 633 nm, 561 nm, 375 nm and 405 nm.  The near UV (375 nm) laser use the same path as violet (405 nm) laser 375nm and cannot be use simultaneously.

FACSAria III is enclosed by the BioProtect IV ClassII Type A2 biosafety cabinet from Baker Company. The cabinet provides HEPA filtration and airflow control to maximize the protection of the operators from the potentially hazardous samples.

Sorting can be performed into two or four tubes, at three flow rates, with the speed of up to 35,000 events/sec . Most cell types can be accommodated with the use of 130, 100, 85 or 70 micron nozzles. The Automated Cell Deposition Unit (ACDU) makes it possible to sort individual cells into multi-well plates, PCR tubes or microscope slides.  The  system operates on a PC-based Windows XP platform using BD FACSDiva software. 

Location: Room 6-125 Katz Group Centre for Pharmacy & Health Research (KATZ).


Cell Analyzers

Attune NxT (14 color, tubes) | Instrument Configuration

The Attune NxT is a benchtop digital flow cytometer that uses acoustic focusing to allow for extremely high event rates (35,000 events/ second). Equipped with 4 spatially separated lasers (405nm, 488 nm, 561nm, and 637 nm) it is capable of analyzing forward and side scatter and 14 fluorochromes with excellent sensitivity for detection of dim events.  Digital electronics allow automated setup and compensation calculation, as well as post-acquisition compensation.

Location: Room 667 Heritage Medical Research Centre (HMRC).

BD FACS Canto II (BSL1) | Instrument Configuration

The FACSCanto II is a three laser bench-top digital flow cytometer. Equipped with 3 air-cooled spatially separated lasers (488 nm, 633 nm and 405 nm) it is capable of analyzing forward and side scatter and 8 fluorochromes with excellent sensitivity for detection of dim events. The system performs an automated flush between samples to assist in reducing sample-to-sample contamination. FACSLoader with a 40-tube capacity enables automated sample acquisition of a large number of samples.

Location: Room 667 Heritage Medical Research Centre (HMRC).

BD LSR Fortessa-SORP (BSL1) | Instrument Configuration

The LSR-Fortessa SORP is a benchtop digital flow cytometer. Equipped with 5 air-cooled spatially separated lasers (375nm, 405nm, 561nm, 488 nm and 640 nm) it is capable of analyzing forward and side scatter and 18 fluorochromes with excellent sensitivity for detection of dim events.

Location: Room 667 Heritage Medical Research Centre (HMRC).

Amnis ImageStream mkII (BSL1)

The Amnis ImageStream mkII is a bench-top imaging flow cytometer. It is equipped with 3 lasers (488 nm, 642 nm and 405 nm) and is capable of analyzing 9 fluorochromes, side scatter, and bright field images. It is equipped with 20X, 40X, and 60X magnification. Please refer to the table here (ImageStream lasers) for a list of suitable fluorochromes- a table is pasted below for a quick reference. This system operates on a PC-based Windows platform using INSPIRE software.

Location: Room 667 Heritage Medical Research Centre (HMRC).

BD LSR Fortessa (BSL1) | Instrument Configuration

The LSR-Fortessa is a benchtop digital flow cytometer. Equipped with 3 air-cooled spatially separated lasers (405nm, 488 nm, and 633 nm) it is capable of analyzing forward and side scatter and 8 fluorochromes with excellent sensitivity for detection of dim events.  The  system operates on a PC-based Windows XP platform using BD FACSDiva software.  Digital electronics allow automated setup and compensation calculation, as well as post-acquisition compensation.

Location: Room 6-127 Katz Group Centre for Pharmacy & Health Research (KATZ).

BD Fortessa X-20 (BSL2) | Instrument Configuration

The LSR-Fortessa X20 is a benchtop digital flow cytometer. Equipped with 5 air-cooled spatially separated lasers (375 nm, 405 nm, 488 nm, 561 nm, and 633 nm) it is capable of analyzing forward and side scatter and 18 fluorochromes, with excellent sensitivity for detection of dim events. This unit is also contained within a BioBubble to permit analysis of BSL2 samples.  The  system operates on a PC-based Windows 7 platform using BD FACSDiva software.  Digital electronics allow automated setup and compensation calculation, as well as post-acquisition compensation.

Location: Room 6-125 Katz Group Centre for Pharmacy & Health Research (KATZ).